Heterogeneity of smooth-muscle cholinesterase in subcellular fractions.

The differences in percentage inhibition by hexafluorenium bromide [hexamethylene1,6-bis-(9-fluorenyldimethylammonium) dibromide (Mylaxa, Nessler, Mallinckrodt, Germany)] and in polyacrylamide-gel disc-electrophoretic pattern of the hydrolytic activity of acetylthiocholine iodide of different fractions of rat jejunum have been reported by Das & Sayer (1 975). Scaf (1971) in his study on the effects of hexafluorenium on the cholinergic system of the gut used jejunum from both rat and guinea pig. Since, it is well known that the heterogeneity of enzymes is species-related, the present communication presents further results on properties and distribution of cholinesterases in guinea-pig jejunum.